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KMID : 0043320010240030229
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Archives of Pharmacal Research
2001 Volume.24 No. 3 p.229 ~ p.233
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Radiation Technology in the Preparation of Polyethylene Oxide Hydrophilic Gels and Immobilization of Proteases for Use in Medical Practice
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Vereschagin EI
Han DH/Troitsky AW/Grishin OV/Petrov SE/Gulyaeva/Bogdanova LA/Korobeinikov MV
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Abstract
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This papers deals with the development of a technology for making a hydrophilic gel of polyethylene oxide reception in which radiating ability is employed to cause cross- linking of polymers in a water solution. The gel of polyethylene oxide was shown to be nontoxic, contain 5-50% of polymer and be useful in composite medicinal forms along with biologically active substances including Bac.subtilis. Proteases. Proteases immobilized in the gel possess high thermal stability and proteolytic activity and are readily applied in medicine. The effect of immobilifed proteolytic aud glucolytic enzymes of Bac.subtillis (Immozimase) on the warm ischemia- reperfusioh (I/R) which can cause hepatic anti jejunum injury was also studied. These enzymes were immobilized on water-soluble polymer polyethlyene glycol by means of an electron beam. The numbers of degranulaled mast cells as well as sedum ALT after I/R in the group with Immozimafe was decreased to almost half as compared with the control group. Pretreatment with lmmozimase resulted in signiflcant reduction of hepatic and gut neutrophil accumulation as compared wish control animals. It was concluded that lmmozimase has a protective effect for hepatic and gut ischemia/repeffusion, and this effect seems to be associated with prevention of leukocyte accumulation.
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